Effect of TGF beta and EGF on Airway Epithelial and Mesenchymal Stem Cell Migration
Joanna Weber, Mirza Zain Baig, Faiz Bhora.
Health Quest, Danbury, CT, USA.
OBJECTIVE: Growth factor treatments have been proposed in tissue engineering application to speed up the migration of the host's cells to the implanted constructs. In the trachea, two main cell types of concern are epithelial cells, which can migrate from adjacent airway mucosa, and mesenchymal stem cells (MSC), which can be mobilized from stem cell niches.
METHODS: Epithelial: Differentiated airway epithelium was grown in air-lift culture in cell culture inserts. Tissue constructs were removed from the inserts, affixed to 6-well plates, and cultured for 48 hours. Media was supplemented with 10ng/mL TGFβ, 1ng/mL EGF, or saline. Migration was assessed at 24 and 48hrs by microscopy. MSC: Cells were added to the tops of 8Ám pore trans-well filters. Media at the bottom of the filter was supplemented as above and migration to the bottom was assessed after 8 and 24hrs by microscopy.
RESULTS: Supplementation with growth factor increased migration for both MSCs and epithelial cells. Growth factors increased migration by ~2-fold in MSCs (Table). With the addition of growth factors, epithelial migration was observed after only 24hrs, while controls showed no migration until 48hrs (Figure 1).
CONCLUSIONS: Although the signaling mechanism through which TGFβ and EGF affect migration are different, they were both effective in increasing epithelial and MSC migration in vitro. Future experiments will investigate more growth factor concentrations and combinations. Using growth factors in bioactive cell-homing grafts will facilitate the creation of acellular grafts which use the patient's own body as a cell source.
|8hr||7.4 ▒ 0.7||14.0 ▒ 0.6*||20.3 ▒ 0.4*|
|24hr||20.6 ▒2.1||35.2 ▒ 3.5*||34.8 ▒ 2.8*|
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